LOCUS       Exported                3910 bp ds-DNA    circular SYN 10-OCT-2014
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    piG0042_regulator_rhlR_corrected
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 3910)
  AUTHORS   Daniel Gerngross
  TITLE     piG0042
  JOURNAL   Exported 10 Oct 2014 from SnapGene 2.5.0
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3910
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     misc_feature    259..450
                     /note="ROP"
     misc_feature    552..692
                     /note="bom"
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(863..1482)
                     /direction=LEFT
                     /note="pBR322_origin"
     oriT            1502..1610
                     /note="incP origin of transfer"
     CDS             complement(1800..2660)
                     /note="AmpR"
     promoter        complement(2661..2752)
                     /gene="bla"
                     /note="AmpR promoter"
     terminator      2825..2919
                     /note="lambda t0 terminator"
                     /note="transcription terminator from phage lambda"
     promoter        2963..2997
                     /note="const. promoter (J23100)"
     RBS             3006..3017
                     /note="RBS (B0034)"
     CDS             3024..3752
                     /codon_start=1
                     /product="RhlR (no LVA)"
                     /note="rhlR (C0171)"
                     /translation="MRNDGGFLLWWDGLRSEMQPIHDSQGVFAVLEKEVRRLGFDYYAY
                     GVRHTIPFTRPKTEVHGTYPKAWLERYQMQNYGAVDPAILNGLRSSEMVVWSDSLFDQS
                     RMLWNEARDWGLCVGATLPIRAPNNLLSVLSVARDQQNISSFEREEIRLRLRCMIELLT
                     QKLTDLEHPMLMSNPVCLSHREREILQWTADGKSSGEIAIILSISESTVNFHHKNIQKK
                     FDAPNKTLAAAYAAALGLI*"
     terminator      3761..3889
                     /note="directional double term (B0015)"
     terminator      3901..77
                     /gene="Escherichia coli rrnB"
                     /note="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc
       61 tcctgagtag gacaaatccg ccgccctaga cagctgggcg cgcccccgcc gcatccatac
      121 cgccagttgt ttaccctcac aacgttccag taaccgggca tgttcatcat cagtaacccg
      181 tatcgtgagc atcctctctc gtttcatcgg tatcattacc cccatgaaca gaaattcccc
      241 cttacacgga ggcatcaagt gaccaaacag gaaaaaaccg cccttaacat ggcccgcttt
      301 atcagaagcc agacattaac gcttctggag aaactcaacg agctggacgc ggatgaacag
      361 gcagacatct gtgaatcgct gcacgaccac gctgatgagc tttaccgcag ctgcctcgcg
      421 cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct
      481 tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc
      541 gggtgtcggg gcgcagccat gacccagtca cgtagcgata gcggagtgta tactggctta
      601 actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc
      661 acagatgcgt aaggagaaaa taccgcatca ggcgctcttc cgcttcctcg ctcactgact
      721 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
      781 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
      841 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
      901 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
      961 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     1021 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     1081 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     1141 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     1201 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     1261 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga
     1321 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     1381 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     1441 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggccggcct
     1501 acggccagcc tcgcagagca ggattcccgt tgagcaccgc caggtgcgaa taagggacag
     1561 tgaagaagga acacccgctc gcgggtgggc ctacttcacc tatcctgccc ggctgacgcc
     1621 gttggataca ccaaggaaag tctacacgaa ccctttggca aaatcctgta tatcgtgcga
     1681 aaaaggatgg atataccgaa aaaatcgcta taatgacccc gaagcagggt tatgcagcgg
     1741 aaaaggacaa gggtcgtcca aaaaaaaagg ctccaaaagg agcctttaat tgtatcggtt
     1801 taccaatgtt taatcaggct cgcgccaatt tccgcaatct gacggttacg ttcatccatc
     1861 gtcgcctggc tgccggtggt ataaatcacc acaatacggc tcggtttacc atccgggccc
     1921 agcgccgcaa taatgccacg gctgccacgt tcgcccgcac cgcttttatc cgcaataaac
     1981 cagccagccg gcagcgcgct acgcagcagc ggacccgcca ctttatccgc ttccatccaa
     2041 tcaatcagtt gctggcggct tgccagggtc agcagctcac ccgtcagcag tttacgcagg
     2101 gtggtcgcca ttgctgccgg catggtggta tcacgttcat cgttcggaat cgcttcgttc
     2161 agttccggtt cccaacgatc cagacgggtc acatgatcgc ccatgttatg cagaaacgcg
     2221 gtcagttctt tcggaccgcc aatggtcgtc agcagcaggt tcgccgcggt gttatcgctc
     2281 atggtaatcg ccgcgctgca cagttcacgc acggtcatgc catcggtcag atgtttttcg
     2341 gtcaccgggc tatattccac cagatcgttc tggctataat gaatacgacg gcccagttgt
     2401 tcctggcccg catccacacg gctcagaacc gcaccgcaca gcagcacttt aaaggtgctc
     2461 atcatcggaa aacgttcttc cggacgaaaa gattccagaa ttttgccgct gttcagatcc
     2521 agttcaatat agcccacgcg cgcacccagt tgatcttccg catctttcac tttcaccagg
     2581 gtttccggat gcgcaaacac cggcaggcaa aacgccgcaa aaaacggaat cagcgccaca
     2641 cgaaaatgct gaatgctcat actcttcctt tttcaatatt attgaagcat ttatcagggt
     2701 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggaa
     2761 ttaaaaaaaa gcccgctcat taggcgggct actaatttaa atcgtaatta ttggggaccc
     2821 ctggattctc accaataaaa aacgcccggc ggcaaccgag cgttctgaac aaatccagat
     2881 ggagttctga ggtcattact ggatctatca acaggagtcc aagactagtc gccagggttt
     2941 tcccagtcac gacgcggccg cattgacggc tagctcagtc ctaggtacag tgctagctac
     3001 tagagaaaga ggagaaatac tagatgagga atgacggagg ctttttgctg tggtgggacg
     3061 gtttgcgtag cgagatgcag ccgatccacg acagccaggg cgtgttcgcc gtcctggaaa
     3121 aggaagtgcg gcgcctgggc ttcgattact acgcctatgg cgtgcgccac acgattccct
     3181 tcacccggcc gaagaccgag gtccatggca cctatcccaa ggcctggctg gagcgatacc
     3241 agatgcagaa ctacggggcc gtggatccgg cgatcctcaa cggcctgcgc tcctcggaaa
     3301 tggtggtctg gagcgacagc ctgttcgacc agagccggat gctctggaac gaggctcgcg
     3361 attggggcct ctgtgtcggc gcgaccttgc cgatccgcgc gccgaacaat ttgctcagcg
     3421 tgctttccgt ggcgcgcgac cagcagaaca tctccagctt cgagcgcgag gaaatccgcc
     3481 tgcggctgcg ttgcatgatc gagttgctga cccagaagct gaccgacctg gagcatccga
     3541 tgctgatgtc caacccggtc tgcctgagcc atcgcgaacg cgagatcctg caatggaccg
     3601 ccgacggcaa gagttccggg gaaatcgcca tcatcctgag catctccgag agcacggtga
     3661 acttccacca caagaacatc cagaagaagt tcgacgcgcc gaacaagacg ctggctgccg
     3721 cctacgccgc ggcgctgggt ctcatctaat aatactagag ccaggcatca aataaaacga
     3781 aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc
     3841 tactagagtc acactggctc accttcgggt gggcctttct gcgtttataa ttaaaggcat
     3901 caaataaaac
//
